天疱疮患者外周血单一核细胞IL-6受体TNFα受体mRNA的表达

Expression of IL-6 and TNFα receptor mRNA in peripheral blood mononuclear cells from patients with pemphigus

目的:研究天疱疮患者外周血单一核细胞(PBMCs)促炎性细胞因子受体mRNA的表达及其意义。方法:用逆转录-聚合酶链反应(RT-PCR)方法,对37例寻常型天疱疮患者及30例健康对照者PBMCs上IL-6受体、TNFα受体mRNA进行检测。结果:所有天疱疮患者及健康对照者均可检测到IL-6受体及TNFα受体mRNA,IL-6受体mRNA表达量在患者组与对照组之间无显著性差异,TNFα受体mRNA的表达量在患者组为0.50±0.19,对照组为0.22±0.10,患者组的表达量显著高于对照组(P<0.001)。结论:天疱疮患者PBMCs的TNFα受体mRNA表达升高,可能在天疱疮的发病机制中起一定的作用。

Objective： To determine the expression of proinflammatory cytokine receptors mRNA in peripheral blood mononuclear cells in patients with pemphigus vulgaris and to explore its significance. Methods： RT - PCR method was applied to detect the expression of IL- 6 and TNFα receptor mRNA in peripheral blood mononuclear cells from 37 patients with pemphigus vulgaris and 30 heahhy volunteers. Results： IL - 6 receptor mRNA was detected in all patients and all volunteers and no significant difference was found in the expression of IL- 6 receptor mRNA between the patients aod the volunteers. In contrast, the expression of TNFα receptor mRNA （0.50 ± 0.19） in patients with pemphigus vulgaris was signifieantly higher than that in healthy volunteers （0.22 ± 0.10, P 〈 0.001）, Conclusion： The results suggest that TNFα receptor may play a role in the pathogenesis of pemphigus.