光损伤早期视网膜感光细胞的病理改变

Pathological changes of photoreceptors at the early stage of light injury

目的:探讨光损伤早期视网膜感光细胞病理改变的特征。方法:健康无眼病SD大鼠15只,随机分为3组,其中1组不接受光照作为正常对照组,另外2组为实验组,分别以2800Lux强度白光持续照射3h和6h造成视网膜光损伤,采用透射电镜观察视网膜感光细胞的病理改变。结果:持续光照6h大鼠的视网膜感光细胞出现显著的病理改变,主要表现为核固缩、染色质边集、核膜内陷以及线粒体和外节膜盘正常结构的破坏。结论:光损伤早期视网膜感光细胞的超微结构即受到破坏并发生凋亡。

Aim ： To investigate the pathological features of photoreceptors at the early stage after light-induced retina injury, Methods： Fifteen SD rats without any eye disease were randomly allocated into 3 groups, One group which was not exposed to light was taken as normal control, The other 2 groups （ experimental groups） were induced retinal light injury by exposed to constant white light with intensity of 2800 Lux for 3 h or 6 h, respectively. The pathological changes of photoreceptors were observed under transmission electron microscope. Results： After 6 h exposure, obvious pathologic changes including nuclear shrinkage, chromatin margination, nuclear notch and the destruction of the mitochondria and outer segment membrane disc were observed in the photoreceptors. Conclusion： At the early stage of light-induced retina injury, photoreceptors develop uhrastructural destructions and result in apoptosis.