纤维素硫酸酯体内抗凝血活性的实验研究

Anticoagulant activity of cellulose sulfate in vivo

目的:研究纤维素硫酸酯对动物体内的抗凝血活性。方法:用试管法测凝血时间(CT),用凝固法测定活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)和凝血酶时间(TT),以肝素和硫酸化右旋糖酐为阳性对照,考察其抗凝血活性的量效性和时效性。结果:纤维素硫酸酯能显著延长CT,APTT和TT,且呈剂量依赖性,但在所试剂量范围内对PT无明显影响;其抗凝血活性在给药2h时达到最大。结论:纤维素硫酸酯具有明显抗凝血作用,强于现有同类抗凝血药硫酸化右旋糖酐,略低于150IU·mg-1的肝素。

Objective：To assess the anticoagulant activity of cellulose sulfate in vivo. Methods： Coagulation time （CT） was measured using a test tube method. Activated partial thromboplastin time （APTT）, thrombin time （TT）, and pro-thrombin time （PT） were monitored via a coagulation method. Heparin and dextran sulfate were used as positive controls. Results： CT, APTT and TT were significantly prolonged by cellulose sulfate in a dose-dependent manner. No significant difference in PT was observed. The maximal anticoagulant potency achieved in 2 hours after the administration of cellulose sulfate. Conclusion： Cellulose sulfate presents an obvious anticoagulant activity. The anticoagulant potency of cellulose sulfate was more powerful than dextran but less than heparin of 150 IU/mg.