HPLC法测定景天加灵片中红景天苷含量

HPLC determination of rhodioloside in Jingtian Jialing tablets

目的:建立HPLC法测定景天加灵片中红景天苷的含量.方法:采用DIAMONSIL C18(250 mm×4.6 mm,5μm)为色谱柱, 以乙腈-0.1%磷酸溶液(5.3:94.7) 为流动相, 流速为1.0 mL·min-1,检测波长280 nm, 柱温30℃.结果: HPLC法测定的线性范围为0.102～1.524 μg ,r=0.999 8;精密度0.32 %(n=5);平均回收率为99.9%.结论:采用HPLC测定景天加灵片中红景天苷含量的方法简便,快速.

Objective： To establish a HPLC method for the determination of rhodioloside in Jingtian Jialing tablets.Methods：The HPLC separation was performed on a DIAMONSIL C18 column （250 mm×4.6 mm, 5μm） with column temperature at 30℃, a mobile phase consisted of acetontrile-0.1% H3PO4 （ 5.3 ： 94.7） with a flow rate of 1.0 mL· min^-1, and a UV detection at 280 nm. Results： The calibration curve was linear in the concentration range of 0.102 - 1.524μg, （ r = 0.999 8） with a RSD of 0.32% （ n = 5）. The mean recovery rate was 99.9 %. Conclusion：This simple, rapid and specific HPLC method to determine rhodioloside in Jingtian Jialing tablets is achieved.