应用Phenyl Bio-Sephar FF进行辣根过氧化物酶精分离的研究

辣根过氧化物酶是一种广泛应用于临床医学诊断以及抗体-抗原研究领域的常用酶。不同纯度和活性要求的酶常常被用于满足不同的应用领域。我们采用phenyl疏水介质填料对该酶进行分离纯化研究,并且通过一次分离收集不同的部分获得不同纯度和活性要求的酶。同时,我们发现采用phenyl疏水介质填料对于辣根过氧化物酶有专一性的捕捉效能,并且可以通过硫酸铵浓度的梯度变化轻易地从柱上解析。这种新型填料的应用和工艺带来了简洁经济的生产流程。

Horseradish Peroxidase is one of kind enzyme, which is widely used in medical diagnostic reagents area and antigenantiboby research scope. Different purity of HRP products meet the requirement of different application. We use phenyl Bio-Sephar media to produce different purity enzymes in one purification step and found this media can capture HRP specifically, the bonded HRP can be eluted by（NH4）2SO4 easily. The purification process is simple and economic.