口腔鳞癌与正常黏膜中细胞角蛋白基因13的表达

Expression and significance of cytokeratin 13 gene in oral squamous cell carcinoma

目的:研究细胞角蛋白基因13(cytokeratin13,CK13)在口腔鳞状细胞癌(oralsquamouscellcarcinoma,OSCC)组织中的表达及意义。方法:用RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常黏膜中CK13mRNA的表达,同时用免疫组化方法检测CK13蛋白在OSCC及正常黏膜标本中的表达。所得数据采用Fisher确切概率计算法进行统计学处理。结果:RT-PCR检测结果表明,CK13mRNA在27例OSCC中的表达较其对照黏膜表达下调,平均下调4.2倍;免疫组化染色结果显示,CK13蛋白在正常口腔黏膜与肿瘤组织之间、高分化OSCC与中、低分化OSCC之间的表达差异具有统计学意义(P<0.05)。结论:在OSCC的发生、发展中,CK13基因的转录和表达水平发生明显变化,可能存在转录后水平的表达调控。CK13在OSCC分类诊断中有应用价值。

PURPOSE： To investigate the expression and its significance of cytokeratin 13 gene in oral squamous cell carcinoma （OSCC）. METHODS： Differential CK13 gene expression between 30 cases of paired normal and cancerous tissues were detected by reverse transcription-PCR （RT-PCR）, and immunohistochemistry was conducted using monoclonal antibody against CK13 in them. The data were analyzed with SAS6.12 software for Fisher＇s exact test. RESULTS： RT-PCR results demonstrated that CK13 gene mRNA was upregulated in 27 cases of normal tissues than in paired cancerous samples, by 4.2-fold in total; and immunostain results revealed that expression of CK13 protein between normal oral squamous cell and OSCC,high differentiated OSCC and poor differentiated ones were significantly decreased （P〈0.05）. CONCLUSIONS： CK13 gcnc might play an important role in OSCC carcinogenesis and be regulated at posttranscriptional levels. Further research will contribute to confirm its role and mechanism. Supported by National Natural Science Foundation of China （ No： 30171014,30330580 ）, Research Fund of Science and Technology Bureau of Shanghai Municipality （ No： 036505013,034107002 ） and Shanghai Leading Academic Discipline Project （Y0203）.