中国汉族牙周炎患者的Fcγ RⅢb基因型分析

Analysis of FcγRⅢb genotype of periodontal disease in the Han nationality in China

目的:研究FcγRⅢb基因型与中国汉族人群侵袭性牙周炎、慢性牙周炎易感性的关系。方法:参照1999年10月牙周病分类国际研讨会制定的标准选择病例,实验分为3组,AgP组23例,CP组20例,Cont组22例。提取受检者静脉血白细胞DNA,用PCR扩增和DNA测序的方法检测FcγRⅢb基因型,以χ2检验比较各实验组基因型的差异。结果:在各实验分组中FcγRⅢb-NA1、-NA2基因(旧定义)分布频率总和小于1,且不含-SH、-NANULL基因型,但存在新的NA1、NA2基因多态性。各基因多态性在组内及组间分布无规律(P!0.05),无统计学差异。NA1、NA2(新定义)在本实验的汉族人群中分布频率为0.77和0.23。结论:本实验的汉族人群中FcγRⅢb包括NA1、NA2基因型,同时还存在其他新的基因多态性(-NA1*01b、-NA1*02a、-NA1*02b、-NA1*03a、-NA1*03b、-NA2*02、-NA2*03、-NA2*04)。FcγRⅢb可能不完全是中国汉族人群牙周病的易感因素。

PURPOSE： The purpose of the present study was to compare FcγRⅢb genotype of aggressive periodontitis （AgP） with chronic periodontitis （CP） and control group （Cont） in the Han nationality in China. METHODS： The classification, which was applied in this study, was presented and discussed at the 1999 International Workshop for the Classification of the Periodontal Disease organized by the American Academy of Periodontology （AAP）. 23 patients with aggressive periodontitis, including 20 with chronic periodontitis according to the established clinical criteria, and 22 matched healthy controls was included in this study. Peripheral blood samples were taken from all patients and controls, then DNA was isolated from blood using DNA purification kit. And FcγRⅢb genotyping was determined by PCR and DNA sequencing. The different results of genotype in all three groups were analysed by X^2test. RESULT： FcγRⅢb- NA1, -NA2（old definition） frequencies didn＇t equal 1.00 in all 3 groups, and also without - SH,-NANULL genotype. At the same time, many new allele of NA 1 and NA2 were found in all groups. However, there were no rules of new alleles in each group and between the groups, no statistical significance as well. The -NA 1 of FcγRⅢb （new definition） frequency was 0.77 ,and that of-NA2 （new definition） was 0.23 in this study. CONCLUSION： Including FcγRⅢb-NA1 and - NA2, five alternate allele of-NA1 （-NA1＊01b, -NAI＊02a, -NA1＊02b, -NA1＊03a and-NA1＊03b） and three alternate allele of-NA2（-NA2＊02, -NA2＊03 and -NA2＊04）were described. The results suggest that FcγRⅢb may not be a risk indicator for the susceptibility of periodontitis in the Han nationality of China. Supported by Tenth Five-Year Key Project of Ministry of Science and Technology of China （2004BA720A26）.