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胸膜肺炎放线杆菌的实时荧光PCR检测 被引量：3

The Detection of Actinobacillus pleuropneumoniae by Real-time PCR
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摘要 Primers FP/ RP and TaqMan-MGB Probe were designed from sequence of apxIVA gene specific to all serotypes of Actinobacillus pleuropneumoniae. A real-time PCR method was developed for detecting Actinobacillus pleuropneumoniae, which can amplify all strains of APP. The sensitivity is 12fg DNA or 5.1CFU bacteria. This method can be directly used for detection of APP without DNA extraction. Primers FP/ RP and TaqMan-MGB Probe were designed from sequence of apxlVA gene specific to all serotypes of Actinobacillus pleuropneumoniae. A real-time PCR method was developed for detecting Actinobacillus pleuropneumoniae, which can amplify all strains of APP. The sensitivity is 12fg DNA or 5.1CFU bacteria. This method can be directly used for detection of APP without DNA extraction.
作者 李树清 易建平 李健 胡永强 王巧全 陈志飞 周筱华 潘晓钟 罗满林 陈敏
机构地区 上海出入境检验检疫局 华南农业大学兽医学院
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2005年第9期969-973,共5页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 上海市科委标准专项资助项目(02DZ05026)
关键词 胸膜肺炎放线杆菌 实时荧光PCR 检测 Actinobacillus pleuropneumoniae real-time PCR detection
分类号 S852.61 [农业科学—基础兽医学]
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参考文献10

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二级参考文献13

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二级引证文献11

畜牧兽医学报
2005年 第9期

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