Au@SiO_2修饰电极的制备及对Cyt c的直接电化学研究

Preparation of the Au@SiO_2 Modified Electrode and Its Application to the Direct Electrochemistry of Cytochrome C

研究Au@SiO2复合纳米材料修饰电极的制备方法及其对细胞色素c(Cytc)的电催化行为.Cytc在该修饰电极上表现出一对氧化还原峰,且在1.0×10-7～2.0×10-5mol/L浓度范围内.Cytc峰电流与浓度呈良好的线性关系,检测下限为5.0×10-8mol/L(S/N=3).还应用紫外可见光谱考察了Au@SiO2复合纳米材料的生物兼容性,结果表明,Au@SiO2材料为Cytc提供了一个“近水”的微环境,使其在该修饰电极表面不易变性,因而具有良好的生物相容性.

Au@SiO2 composite nanoparticles were prepared. By controlling synthesis conditions, the thickness of the silica shell could be varied in the range of tens to several hundred nanometers. Coupled with chitosan （CHIT）, Au@SiO2/CHIT/GC modified electrode was fabricated. The electrode was applied to the determination of cytochrome c （Cyt c）. The results of the experiments indicated that the currents of Cyt c were linear to its concentrations over the range from 1.0 × 10^-7 to 2.0 × 10^-5 mol/L and the calculated detection limits （S/N= 3） was 5.0 × 10^-8 mol/L. Further work could be done to apply this electrochemical method to preparing biosensor and detecting biologic samples.