摘要
目的:探讨Troglitazone对小鼠I型葡萄糖载体(mGLUT1)表达的影响。方法:采用分子克隆技术,将过氧化物酶体增殖物受体γ(PPARγ)和维甲酸类受体X(RXRα)及mGLUT1 cDNA分别克隆到表达载体pCMX和pGL3b上。PPARγ与RXRα及mGLUT1克隆载体转染NIH 3T3细胞,处理或不处理Troglitazone,应用荧光素酶活性测定法及RNA印迹等方法测定Troglitazone对mGLUT1重组体荧光素酶活性调节及对mRNA表达的影响。结果:Troglitazone可激活mGLUT1重组体荧光素酶的活性,并且可增加mGLUT1的mRNA表达水平。结论:Troglitazone可增强mGLUT1的表达,可能参与PPARγ对mGLUT1的调节过程。
Objective：To understand the possibility of effect of troglitazone on mGLUT1 expression. Methods： PPARγ , RXRα and mGLUT1 cDNA clone into pCMX and pGL3b expression vector. The recombinant DNA transfected into NIH 3T3 cells, treated trogiltazone and confirm the mGLUT1 activity and mRNA expression by the trogiltazone through PPARY activation with luciferase assay and Northern blot. Results： Troglitazone activates the mGLUT1 activity and increase the mGLUT1 mRNA expression through PPARγ activation. Conclusion： Troglitazone binds to PPARy and activates mGLUT1 expression.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2005年第10期949-950,共2页
Lishizhen Medicine and Materia Medica Research
基金
吉林省教育厅基金资助项目(No.2004-004)
