摘要
目的:纯化桑叶多糖并测定其含量。方法:采用10%三氯乙酸、大孔树脂初步纯化桑叶多糖,采用硫酸-蒽酮法对其含量进行测定。结果:用三氯乙酸除蛋白的方法是可行的;桑叶中多糖的平均含量为2.62%,平均回收率为98.81%,RSD为2.08%。结论:该法可作为桑叶中多糖的含量测定方法。
Objective：To purify and determine the contents of polysaccharide in Morus alba L. Methods： The polysaccharide were preliminarily purified by 10% trichloroacetic acid and resin. The contents of polysaccharide were determined by sulfuric acid anthrone method and detected at 620nm by UV/Visible spectrophotometry. Results： The average contents of polysaccharide in Morus alba L. was 9.62%. The recovery rate was 98.81% with 2.08% of RSD（n=5）.Conclusion：The sulfuric acid anthrone method is reasonable.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2005年第10期961-962,共2页
Lishizhen Medicine and Materia Medica Research
基金
江苏省科技攻关基金项目(No.BE2004352)
关键词
桑叶多糖
提取纯化
硫酸-蒽酮法
Morus alba L
Polysaccharide
Purification
Sulfuric acid anthrone
