植物性雌激素三羟异黄酮对脑缺血大鼠学习记忆的影响

The effect of genistein on the learning and memory in cerebral ischemia rats

目的:观察植物性雌激素三羟异黄酮(GST)对脑缺血大鼠学习记忆的影响。方法:雌性大鼠,行双侧卵巢切除手术后,皮下注射三羟异黄酮治疗,10天后结扎双侧颈总动脉,制备脑缺血动物模型。2个月后,用水迷宫法测定大鼠学习记忆能力的改变,并取海马组织作超薄切片电镜分析。结果:①水迷宫测试结果:GST组与去卵巢对照组比较,游完全程的时间明显缩短,错误反应次数明显减少。②形态学观察:去卵巢对照组海马神经元超微结构有明显损害,而GST组能明显改善海马神经元的损伤。结论:GST通过对脑缺血动物脑神经元保护作用,提高卵巢去势大鼠学习记忆的能力。

Objective：To observe the effect of genistein on the learning and memory in cerebral ischemia rats. Method： Adult female rats were bilaterally ovariectomized, and genistein was injected as a curative. After 10 days,The cerebral ischemia was induced by the bilateral common carotid artery ligated. 2 months later, all rats underwent water maze behavior testing; Tissue specimens for each group were harvasted from the hippocampus area for electonmicroscopy studies.Result： Results of water maze behavior testing： In the genistein group, full-course swimming time shortened and number of errors reduced compared with the ovariectomized control group. The hippocampus uhrastructure has abnormal change in the ovariectomized control group. But in the genistein group, it was lesser. Conclusion：Genistein can increase the learning and memory in cerebral ischemia rats.