摘要
本实验首先确定潮霉素浓度对香菇和平菇菌丝和原生质体再生的影响。其次进行了温度和时间对平菇原生质体灭活效果的研究。然后利用香菇和平菇再生原生质体对潮霉素抗潮霉素的差异,结合平菇原生质体灭活为融合子筛选依据,在融合剂诱导下获得4株融合子。
Hygromycin B resistance was studied on the mycelium and protoplast of Lentinula edodes and Pleurotus ostreatus. Test was done on inactivation of protoplast of P. ostreatus by temperature and duration. The protoplasts were prepared and fused with fusogen （PEG） after inactivation of protoplasts of Pleurotus ostreatus at 60℃ for 2h. Four fusants were obtained in the selection medium containning hygromycin B.
出处
《中国食用菌》
2005年第5期21-22,27,共3页
Edible Fungi of China
关键词
香菇
平菇
原生质体融合
融合子
潮霉素
Lentinula edodes
Pleurotus ostreatus
Protoplast fusion
Fusant
Hygromycin B
