高效液相色谱法测定阿莫西林/氟氯西林钠胶囊溶出度

Detection for amoxillin/flucloxacillin Capsules by HPLC

目的:建立阿莫西林/氟氯西林钠胶囊溶出度测定方法。方法:采用转篮法,以水为溶剂,转速为100r/min。高效液相色谱法,以C18柱为色谱柱,以用磷酸调pH值至2.6的0.1mol/L磷酸二氢钾-0.018mol/L十二烷基硫酸钠-甲醇-乙腈(275∶275∶200∶250)溶液为流动相,检测波长为225nm。结果:阿莫西林与氟氯西林的线性范围均为0.03～0.60mg/mL,r=0.9999;平均加样回收率阿莫西林为99.8%,RSD=0.8%(n=9),氟氯西林为99.9%,RSD为1.3%(n=9)。结论:高效液相色谱法简便、准确、灵敏度高。

Objective： To establish the method for dissolution of amoxillin/flucloxacillin capsules by HPLC. Methods： An C18 column was used. The mobile phase was 0.1mol/L KH2PO4-0.018 mol/L SDS - methanol - acetonitrile （275 ： 275 ： 200 ： 250）, using phosphonic acid adjust pH to 2.6. The wavelength of UV detector was 225 nm. Results： The average recovery of amoxillin was 99.8% （RSD=0.8%, n=9）, the average recovery of flucloxacillin was 99.9% （RSD=1.3%, n=9）. Conclusion：The method was convenient and accurate.