Survivin、Caspase-3蛋白在非小细胞肺癌的表达及其相关性研究

EXPRESSION OF Survivin AND Caspase-3 IN HUMAN NON-SMALL CELL LUNG CANCER AND THEIR RELATIONSHIP

目的研究Survivin、Caspase-3蛋白在非小细胞肺癌(NSCLC)组织的表达及其生物学意义,并探讨其相关性。方法用免疫组化(2步法)染色技术对47例NSCLC组织石蜡标本进行Survivin、Caspase-3蛋白表达的检测和分析。结果Sur-vivin、Caspase-3在非小细胞肺癌中阳性表达率为59.57%、57.45%;与性别、年龄、组织学分型、病理分级、TMN分期、淋巴结转移无明显相关(P>0.05);Survivin与Caspase-3蛋白的表达呈负相关(U 2.325;P<0.05)。结论Survivin蛋白在非小细胞肺癌中表达上调,提示其通过抑制细胞凋亡,在肺癌癌变发生、发展中起重要作用,可能成为肺癌基因治疗的新靶点;Sur-vivin通过与激活的Caspase-3结合,抑制其活性,从而阻止细胞凋亡,其阳性表达亦预示肿瘤有较高的侵袭性和不良预后。

Objective To investigate the expression and distribution of Survivin and Caspase -3 in human non-small cell lung cancer（NSCLC）, and to evaluate the relationship between Survivin and Caspase-3. Methods The expression of Survivin and Caspase-3 in 47 patients with NSCLC were examined by immunnohistochemical methods, and the relationship between them and clinicopathological parameters were analyzed. Results The positive rates of Survivin and caspase-3 in NSCLC were 59.57 % and 57.45 % respectively. The positive expression levels of Survivin and Caspase-3 were not associated with gender, age, histological differentiation, subtype, TMN stages or lymph node metastases（ P 〉0.05）. There was a negative correlation between Survivin and Caspase-3. Conclusion Survivin may play an important role in the pathway of carcinogenesis and progression of NSCLC and it may be identified as a new therapeutic target. Inhibition of the Caspase-3′ s activation is the anti-apoptosis mechanism of Survivin. The up-regulation expression of Survivin predicts more invasion and poorer prognosis.