肾移植受者的细胞因子及其受体单核苷酸多态性与术后感染的相关性

Impacts of recipient’s SNP of cytokine and cytokine receptor on the incidence of infection after renal transplantation

目的探讨肾移植受者的某些细胞因子及其受体基因多态性与术后感染的相关性。方法采用自行研制的细胞因子及其受体单核苷酸多态性检测芯片,分析129例肾移植受者的肿瘤坏死因子(TNF-α)、白细胞介素10(IL-10)、转化生长因子(TGF-β1)、IL-4、IL-6及其受体的21个位点的基因多态性分布情况,并按照患者术后是否发生感染进行分组比较。结果5种细胞因子及其受体单核苷酸多态性在感染组和非感染组中分布明显不同,分别是基因型IL-6R(-183GG、G/A)、IL-10(-824C/T,-597C/A)及TNF-α(-308GG、G/A);等位基因为IL-10R1(1112G/A)、IL-6R(-183G/A)、IL-4R(1902A/G)、TNF-α(-308G/A)及TGF-β1(+869T/C)。结论基因型IL-6R(-183GG)、IL-10(-824C/T,-597C/A)及TNF-α(-308GG),等位基因IL-4R(1902A)、IL-6R(-183G)、IL-10R1(1112G)、TNF-α(-308G)及TGF-β1(+869C)是肾移植后感染的易患因素;而基因型IL-6R(-183G/A)和TNF-α(-308G/A)可能为移植后感染的非易患因素。

Objective To explore the influence of renal allograft donor＇s and recipient＇s SNP of recipient cytokine and cytokine receptor on the infection after renal transplantation and to provide some useful information for preventing and managing infection. Methods 129 cases of cadaveric renal allograft recipients were divided into infection group and no infection group. The distribution of 21 polymorphisms in cytokines and cytokine receptors gene were compared between two groups by oligonucleotide array. Previous positive gene polymorphisms were compared between infection group and no infection group. With the help of SPSS 11.5 software, association was assessed using Krusakal Wallis test where appropriate. Results The frequency of gene distribution was significantly different between the infection group and the no infection group as follows： the genotype IL-6R （ - 183G/A, GG）, IL- 10 （ - 824C/T, - 597C/A）, TNF-α （ - 308GG, G/A）, and the allele IL-10R1 （1112G/A）, IL-6R （ - 183G/A）, IL-4R（1902A/G）, TNF-α （ - 308G/A）, TGF-β1 （ ＋ 869T/C） respectively. Conclusion The susceptibility of infection after renal transplantation may be predicted by the SNP of recipient cytokine and cytokine receptors such as these genotypes IL-6R （- 183GG）, IL-10 （- 824CT, - 597CA）, TNF-α（ - 308GG）, and the allele IL-4R（1902A）.