HCV-1b型HVR1区准种变异与干扰素疗效的关系

Relationship Between Hypervariable Region 1 of Hepatitis C Virus Quasispecies Complexity and Response to Interferon Therapy

目的研究丙型肝炎病毒基因1b(HCV-1b)HVR1区准种变异与干扰素疗效的关系。方法选取HCV-1b型慢性丙型肝炎患者20例,在干扰素治疗前作HVR1区准种变异测定,同时进行HCV RNA定量分析,比较其与干扰素疗效的关系。结果7例干扰素治疗有效者的异源双链杂交电泳分析大部分<3带,平均为(2.37±0.89)带;而13例干扰素治疗无效者的结果均>3带,平均(4.75±0.92)带;两者比较差异有非常显著性(P<0.01);且准种数量与治疗前HCV RNA水平呈显著正相关(r=0.35,P<0.01)。结论干扰素的疗效与HVR1准种变异密切相关,有效者准种变异的程度明显低于无效者,即患者治疗前HCV准种变异较少者,HCV病毒易于被清除;采用异源双链杂交电泳法(HMA)检测HVR1区准种变异,对预测慢性丙型肝炎干扰素疗效有一定的实用价值。

OBJECTIVE To investigate the relationship between hepatitis C virus （HCV） hypervariable region 1 （HVR1） quasispecies complexity and the effectiveness to interferon （IFN） therapy in patients with HCV lb infection. METHODS Dynamic changes of HCV quasispecies before and after IFN therapy were detected by the method of heteroduplex mobility analysis （HMA）. RESULTS The complexity of HVR1 quasispeeies in responders was less than that in nonresponders before IFN treatment. Numbers of HMA bands of HVR1 were decreased after IFN therapy in most patients. CONCLUSIONS The low complexity of HCV HVR1 quasispeeies indicates the effectiveness of IFN therapy in patients with HCV lb infection.