516株金黄色葡萄球菌对9种抗生素的耐药性分析

Resistance of 516 Staphylococcus aureus Isolates of to 9 Kinds of Antibiotics

目的了解金黄色葡萄球菌对9种常见抗生素的耐药性,为临床治疗提供依据. 方法按NCCLS纸片扩散方法,测定并判读金黄色葡萄球菌对抗生素的耐药性,并以NCCLS纸片法测定MRSA耐药表型. 结果 516株金黄色葡萄球菌对青霉素的耐药率均约90%,除对万古霉素和复方新诺明外,2004年该菌对监测的9种抗生素的7种药物的耐药率＞57.8%,MRSA菌为64.4%,目前我院未检测到对万古霉素耐药的金黄色葡萄球菌;516株金黄色葡萄球菌MRSA的平均检出率为44.8%;MSSA对青霉素的耐药率高达86.6%,对红霉素耐药率为55.1%,对克林霉素、四环素和复方新诺明的耐药率约20%～30%;MRSA除复方新诺明和万古霉素外,对其他各类药物耐药性明显升高,对环丙沙星耐药增高最明显(77.5%). 结论金黄色葡萄球菌感染发生率及耐药性快速增长, MRSA对各类抗生素多重耐药.

OBJECTIVE To investigate the resistance of Staphylococcus aureus isolated from 2000 to 2004 to nine kinds of antimicrobial agents. METHODS Susceptibility test was performed by disk diffusion method and analyzed by software WHONET 5.3. RESULTS The 516 S. aureus strains showed resistant rates of 90% to penicillin, the percentage of resistance of S. aureus to the nine drugs increased with years, accounted for over 57.8% except for sulfamethoxazole/trimethoprim and vancomycin in 2004. The isolated rate of MRSA was 44.8%. The resistance of MSSA to penicillin and erythromycin was 86.6% and 55. 1%, respectively. None of the isolates showed resistance to vancomycin. CONCLUSIONS There is an increasing trend of isolated rate and resistance in S. aureus. MRSA strains are multidrug resistant.