成纤维细胞上清提取物对表皮细胞增殖迁移的影响

Effect of available components in culture supernatants of fibroblasts on proliferation and migration of human epithelia

目的:在人皮肤成纤维细胞培养上清液中寻找有效的生长因子样活性组分.方法:收集超滤成纤维细胞的培养上清液,采用MTT法、划痕试验检测对人皮肤表皮细胞增殖和诱导迁移的影响.结果:上清液中分子量5～10 KD组和5～30 KD组的试验组对表皮细胞有明显促增殖趋势,而5～30 KD组有较强的促进单层细胞伤口愈合的能力,二者与对照组比较有显著性差异(P＜0.01).结论:人成纤维细胞培养上清液的提取物中含有促进表皮细胞增殖及迁移能力的活性物质,可以尝试用其替代部分条件培养基进行对组织工程皮肤种子细胞的实验研究.

Objective： To find the available and effective components from the culture supernatants of fibroblasts. Method：The supernatants were collected and separated by ultrafiltration. The proliferation of cultured epithelia were mea- sured by MTT method. The migration and enhancement of inducing Ep to wound closure of confluent Ep were observed. Result：The in vitro cellular study showed significant difference between group 3,5 and control group（ P〈 0.01 ）. Conclusion：There are positive factors which are beneficial to the proliferation and migration of cultured epithelia in the part of 5-10 KD and 5-30 KD from the culture supernatants of the human skin fibroblasts. We can partly substitute conditional medium using it to carry through the in-depth study of seed cells of tissue engineering of skin.