核素骨显像在局部应用rh-BMP/bFGF加速山羊下颌骨牵张成骨实验研究中的应用价值

Evaluation the value of radionuclide bone imaging in the use of rh-BMP/bFGF to accelerate bone formation during mandibular distraction osteogenesis in goat

目的:应用核素骨显像观察局部应用rh-BMP/bFGF加速山羊下颌骨牵张成骨的效果。方法∶利用外置式牵张器进行山羊下颌骨牵张成骨。将10只实验用山羊随机分为5组,每组2只,在下颌第2和第3前磨牙之间行骨皮质切开,将含有rh-BMP 15 mg及bFGF 2000 UI的载体胶原膜置入左侧牵张骨折间隙,即刻牵开1 mm以利容纳置入的载体胶原膜。对照侧不加生长因子,牵引速度同实验侧。术后5 d开始牵引,3 mm/d,0.5 mm/次×6次/d,分别于牵张完成当日及第2、4、6、12周时将动物进行放射性核素骨扫描检查。结果:随着时间的延长,在不同周期,实验侧牵张间隙中央出现核素浓聚,强度明显较对照侧强。牵张完成12周:实验侧和对照侧核素浓聚变弱,基本上回复到周围正常骨组织密度水平,两者差异明显减少。结论:局部应用rh-BMP/bFGF后实验侧下颌牵张后新骨生成速度和数量优于对照侧,说明外源性生长因子能够加速下颌骨牵张延长区的骨愈合,核素骨扫描检查在观察牵张成骨的效果中具有较高的应用价值。

Objective： To study the value of ^99mTc-MDP bone scanning in the use of rh-BMP/bFGF to accelerate bone formation during mandibular distraction osteogenesis in goat. Method： 10 goats were divided into 5 groups, two goat in each groups. After bilateral mandibular cortical osteotomy between the second and third premolars, rh-BMP/bFGF were implanted into the distracted region on the left side whereas right side was used as control. Intraoral implant-supported distraction devices were placed. The regenerated callus were harvested and processed for radiographic ECT inspection evaluation were performed at different periods. Result： The result showed that The ratio of new bone formation of rh-BMP/bFGF was significantly higher than that of control side. The uptake of ^99mTc-MdP in rh-BMP/bFGF was higher than that of control side at different time, but there were no significant differences between the two groups at 12th weeks . Conclusion：The rh-BMP/bFGF can accelerate bone formation during mandibular distraction osteogenesis in goat and radionuclide bone imaging is a major method for early assessment of bone distraction osteogenesis.