摘要
研究了一种用于芦丁含量测定与纯度检验的高效液相色谱法(HPLC).该法具有较高精度和良好专属性,适于例行分析.分离在C_8柱上进行,流动相为THF(四氢呋喃)-磷酸缓冲液,检测波长为280nm.检测限为0.5μg/mL,可检出0.05%的杂质,用于某些实际样品分析,结果良好.
Studies on a high-performance liquid chromatographic method were made for purity analysis and microamount quantitation of rutin and its pharmaceutical preparations. The proposed method, with both high precision and /or accuracy and good speeifity and/or selectivity, can be suitably used for routin analysis. Separation was performed on a C-8 column as the stationary phase using phosphate buffer solution modifed by tetrahydrofuran (THF) as a mobile phase as well as uv 280nm as the detecting wavelength. The detection limit was 0. 5μg/mL and the impurities as little quantity as 0. 05% were determined, ff present. The developed method was applied to annlyais of some practical samples with good results.
出处
《分析科学学报》
CAS
CSCD
1996年第1期23-26,共4页
Journal of Analytical Science
基金
国家教委重点资助课题
机械器部重点资助课题
日本政府文部省重点资助课题
湖南省卫生厅重点资助课题
