九种嘌呤碱的毛细管区带电泳分离

Capillary Zone Electrophoresis Separation of Nine Purine Bases

用毛细管区带电泳法分离和测定咖啡碱，９－Ｎ二羟丙基茶碱，可可碱，腺嘌呤，鸟嘌呤、茶碱、黄嘌呤、尿酸和６－巯基嘌呤，在熔硅毛细管（７０ｃｍ×７５μｍｉ．ｄ，有效长度６３ｃｍ）内，使用０．０１ｍｏｌ／Ｌ（ｐＨ＝１０）硼砂缓冲溶液，电压为２５ｋＶ温度为３０℃，真空进样１ｓ，于２６０ｎｍ处检测，可在７ｍｉｎ内分离上述到９种嘌呤碱，理论塔板数达到３．０×１０＾５，检测限达ｆｍｏｌ级，方法用于人尿分析。

This paper reports the separation and determination of caffeine,9-N-dihydroxypropyltheophylline, theobromine, adenine, guanine,theophylline, xanthine, uric acid and 6-mercaptopurine by capillary zone electrophoresis, In an uncoated fused silica capillary(70cm ×75μm i.d., effective length 63 cm), the above mentioned materials can be separated within 7 min in the optimum conditions:0.01 mol/L pH=10 borate buffer,30℃,25 kV,260 nm and 1s load. Efficiency to 3.0×105 the number of theoretical plate is achieved. The detection limit is fmol. The results of urine sample analysis are satisfactory.