摘要
牛红细胞铜锌超氧化物歧化酶(BESOD)在汞电极表面吸附行为的研究钱雯,孟庆国,罗勤慧,沈孟长(南京大学配位化学研究所,配位化学国家重点实验室南京,210093)关键词铜锌超氧化物歧化酶,吸附,循环伏安,双阶跃计时电量超氧化物歧化酶是超氧离子(·O)...
The electrochemical behaviour of bovine erythrocyte superoxide dismutase(BESOD)adsorbed On the mercury electrode surface was reported for the first time by cyclic voltammetry,direct current polarography and double potential-step chronoculoumetry without using mediator.The contribution of diffusion current predominated over adsorption current when the concentration of BESOD was more than 1.0×10 ̄(-4)mol/L. The experimental adsorption isothermal line was in accordance with that calculated by Langmuir equation. The equilibrium constant of adsorptin was 8. 96×10 ̄4 and Gibbs free energy was -28. 2kJ/mol.The molecular area(18. 06 nm ̄2)of BESOD obtained by the electrochemical method, is in good agreement with that(17. 36 nm ̄2)determined by X-ray crystal structural analysis.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第5期687-689,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
超氧化物歧化酶
吸附
循环伏安
牛红细胞
Cu
Zn-superoxide dismutase
Adsorption
Cyclic voltammetry. Double potential-step chronocoulometry