牛红细胞铜锌超氧化物歧化酶（BESOD）在汞电极表面吸附行为的研究

The Electrochemistry Behaviour of Adsorption of BESOD on Mercury Electrode Surface

牛红细胞铜锌超氧化物歧化酶（ＢＥＳＯＤ）在汞电极表面吸附行为的研究钱雯，孟庆国，罗勤慧，沈孟长（南京大学配位化学研究所，配位化学国家重点实验室南京，２１００９３）关键词铜锌超氧化物歧化酶，吸附，循环伏安，双阶跃计时电量超氧化物歧化酶是超氧离子（·Ｏ）...

The electrochemical behaviour of bovine erythrocyte superoxide dismutase(BESOD)adsorbed On the mercury electrode surface was reported for the first time by cyclic voltammetry,direct current polarography and double potential-step chronoculoumetry without using mediator.The contribution of diffusion current predominated over adsorption current when the concentration of BESOD was more than 1.0×10￣(-4)mol/L. The experimental adsorption isothermal line was in accordance with that calculated by Langmuir equation. The equilibrium constant of adsorptin was 8. 96×10￣4 and Gibbs free energy was -28. 2kJ/mol.The molecular area(18. 06 nm￣2)of BESOD obtained by the electrochemical method, is in good agreement with that(17. 36 nm￣2)determined by X-ray crystal structural analysis.