葡萄糖异构酶的固定化及其性质研究

Studies on the Immobilization of glucose Isomerase and the Properties of the Immobilized Glucose Isomerase

用分子沉积法，在多孔三甲胺基聚苯乙烯载体上成功地固定化了双层葡萄糖异构酶。结果表明，这种新固定化酶方法能使单位重量的固定化酶活力及蛋白载量成倍增加，活力达到１２００ＩＵ／ｇ湿胶。与吸附法相比，酶活力提高１倍；其半衰期与吸附法相比则基本相同，为４５ｄ．还确定了最佳固定化条件并测定了固定化酶的性质。固定化酶的最适反应温度比液相酶提高１５℃，而最适ｐＨ值则没有改变。Ｋ＿ｍ值与液相酶的相比有一定程度的增加，稳定性与吸附法相比则基本相同。

In this paper we successfully immobilized bilayer glucose isomerase in porous ptrimethylamine-polystyrene(TMPS)beads by use of a new method of immobilization of enzyme──molecular-deposition.The effects of immobilizing time,the concentration of nature enzyme, the concentration of hexamethylene bis(trimethylammonium bromide), the concentration BCD were investigated.The properties of this immobilized enzyme were also studied. The results obtained show that the activity and the protein loading of per weight bilayer immobilized enzyme are doubled as compared to monolayer immobilized enzyme. The activity of immobilized bilayer glucose isomerase is 1 200 IU/g wet gel.