摘要
研究了重水固氮酶体系中N_2对C_2H_2的还原活性和立体化学产物的影响,发现N_2的存在不仅抑制了C_2H_2的还原活性,而且提高了产物乙烯中反式1.2-二氘代乙烯的相对含量,为证实N_2在固氮酶M簇合物笼内的还原氢化提供了一个有力的证据。
The experiments that the reductive deuteration and products-selectivity of C_2H_2 are affected by N_2 in D_2O-nitgrogenase have been done. It is found that the C_2H_2 reductive activities decreased ca. 80.7%and 89.3%,respectively,with mixtures of C_2H_2and N_2 in the ratios of 1:108.3 and 1:216.7(volume ratio),as compared with ca. 42.6%and 74.2% observed with C_2H_2 in argon(1:65 and 1:108.3,volume ratio) in the absence of N_2;ca. 3.19%and 4.95%trans-2-didutero ethene were observed form FTIR spectra of the ethenes-1,2-d_2 obtained, respectively,with mixtures of C_2H_2 and N_2 in the ratios of1:108.3 and 1:216.7(volume ratio).The results give a strong proof for the conclusion in which N_2 binds inside the cage of M-cluster.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第5期760-763,共4页
Chemical Journal of Chinese Universities
基金
国家基础性研究重大关键项目
关键词
M簇合物
还原
乙炔
固氮酶
氮键合位
N_2-bonding site,M-cluster,C_2H_2 reductive activity,Stereo-selectivity