庆大霉素与3，5－二溴水杨醛的缩合反应及其应用

A Schiff Reaction and Its Application on Spectrophotometric Determination of Gentamycin

本文研究了庆大霉素与３，５－二溴水杨醛缩合成席夫碱的条件及其应用。在加热条件下，庆大霉素与３，５－二溴水杨醛缩合成亮黄色席夫碱，其分子反应比为，庆大霉素＋３，５－二溴水杨醛＝１＋１。据此提出了庆大霉素生物效价总量的光度测定法，庆大霉素在０～１１８．９μｇ／ｍＬ内符合比尔定律。λｍａｘ＝４３０ｎｍ，表现摩尔吸光系数ε＝９．９８×１０￣４Ｌ·ｍｏｌ￣（－１）·ｃｍ￣（－１），本法干扰少，重现性好。

A new sensitive spectrophotometric method for the determination of gentamycin has been studied.It is based on the Schiff reaction,in which 3,5-dibromosalicylaldehyde reacts with gentamycin in acetic-acetate buffer solution to form a yellow compound.The molar ratio of the reaction was found to be 1+1.Its maximum absorption was measured at 430nm with a molar absorptivity of 9. 98×10￣4 L·mol￣(-1)·cm￣(-1).Beer’s law is obeyed in the range of 0～118. 9μg/L for gentamycin.The method has been used to determine gentamycin in semi-finished products with satisfactory results.