高压液相色谱内表面反相填料的发展及其应用

Development and Applications of HPLC Internal Sulface Reversed Phase

内表面反相填料（ＩｎｔｅｒｎａｌｓｕｒｆａｃｅｒｅｖｅｒｓｅｄｐｈａｓｅＩＳＲＰ）是１９８５年首次研制出来的一种高压液相色谱填料，其微孔内表面接疏水基团，外表面接亲水基团，当含蛋白质或其它大分子的样品进入柱子后，蛋白等大分子不被系统保留，而小分子物质则可以进入微孔得到分离。这一填料的研制和发展使血清样品直接进样成为可能，在药物分析方面有独特的优点。本文综述了ＩＳＲＰ的特点、合成方法、工作原理及其应用。

Internal surface reversed phase (ISRP),a kind of high performance liquid chromatographic packing material,was first developed in 1985.ISRP has hydrophobic groups attached to the surface inside the pores and hydrophilic groups attached to the surface outside the pores,With direct injection of samples on the ISRP column, proteins are excluded from the pores and the small molecule substances are retained and separated.The principles,characteristics,preparation method for ISRP and its applications have been reviewed.