摘要
目的:观察具有抗炎、免疫调节等作用的旱莲草水提物对小鼠缺氧存活时间和耗氧量的影响,评价旱莲草的耐缺氧作用。方法:实验于2004-08/12在泰山医学院药学院药物综合实验室完成。33只小鼠随机分为3组:2个实验组(大、小剂量旱莲草水提物组,剂量分别为15和30g/kg)和生理盐水组,每组11只。实验组分别单剂量灌胃给予旱莲草水提物15和30g/kg;生理盐水组单剂量灌胃给予等容量氯化钠注射液30mL/kg。小鼠灌胃给药20min,应用小动物耐缺氧与耗氧量动态变化同步测定装置,测定小鼠缺氧存活时间和耗氧量变化。结果:纳入33只小鼠均进入结果分析。①小鼠10min时的累计耗氧量:小剂量旱莲草水提物组比生理盐水组降低了19.70%;大剂量旱莲草水提物组比生理盐水组降低了32.03%。②小鼠不同时间存活率比较:生理盐水组和大、小剂量旱莲草水提物组50、60、70min时的存活率分别为犤(34%,45%,100%);(18%,18%,60%);(0%,0%,,3%)犦。③小鼠0～5min时耗氧量比较:小剂量旱莲草水提物组比生理盐水组降低了19.05%;大剂量旱莲草水提物组比生理盐水组下降了66.67%。结论:旱莲草水提物能延长缺氧小鼠的存活时间,提高不同时间缺氧小鼠的存活率,降低小鼠不同时间的累计耗氧量,增强小鼠的耐缺氧能力,以大济量旱莲草组(30g/kg)效果明显。
AIM： To observe the effect of aqueous extract from Herba Eclipta on the survival time and oxygen consumption in hypoxic mice, and evaluate the hypoxia tolerance of Herba Eclipta. METHODS： The experiment was finished in the Comprehensive Laboratory of Pharmacology, College of Pharmacy, Taishan Medical University from August to December 2004. Thirty-three mice were randomly divided into three groups and respectively administered with normal saline 30 mL/kg and different dosages of aqueous extract from Herba Eclipta at the dose of 15 g/kg and 30 g/kg. Changes of the survival time and oxygen consumption in mice were mesured. RESULTS： All the 33 mice were involved in the result analysis. Compared with the group of the normal saline （30 mL/kg）, aqueous extract from Herba Eclipta at the dose of 15 and 30 g/kg made the cumulative oxygen consumption respectively reduced by 19.05% and 66.67% in 0-5 minutes and 19.70% and 32.03% in 5-10 minutes. The survival rate of mice with hypoxia in 50 minutes were 34%, 45% and 100%, in 60 minutes 18%, 18% and 60 %, and in 70 minutes 0, 0 and 3% when receiving normal saline and aqueous extract from Herba Eclipta of 15 and 30 g/kg. CONCLUSION： The aqueous extract from Herba Eclipta can reduce the cumulative oxygen consumption in different time and enhance the hypoxia tolerance in mice,especially the large-dose extract.
出处
《中国临床康复》
CSCD
北大核心
2005年第24期168-169,共2页
Chinese Journal of Clinical Rehabilitation
