摘要
采用RAPD技术对遗传学模式生物--果蝇的野生型及7种突变体的遗传差异进行比较研究.结果表明,所选用的37种RAPD引物中,有25种引物扩增谱带清晰,扩增总片断284条,单个引物的扩增片段数为6～15条,平均为11.36条.不同果蝇品种间的遗传差异较小,遗传距离(D)为0.033 4～0.208 9.根据D值,由UPGMA聚类分析绘制了它们的分子进化树.
RAPD （random amplified polymorphic DNA） was used to analyze the genetic diversity among Drosophila. The genetic variance of eight Drosophila was studied. The result showed that：25 of 37 arbitrary primers could amplify clear bands. A total of 284 fragments were obtained. Each primer gave 6～15 bands. The D value between different Drosophila was 0.0334～0.2089.The D value was used to construct a dendrogram by UPGMA.
出处
《河北师范大学学报（自然科学版）》
CAS
北大核心
2005年第5期512-515,共4页
Journal of Hebei Normal University：Natural Science
基金
河北师范大学自然科学基金资助项目(L2004Y08)
