阿托伐他汀对家兔动脉粥样硬化血管细胞粘附分子-1基因表达的影响

Effects of atorvastatin on expression of VCAM-1 in atherosclerotic rabbits

目的探讨家兔动脉粥样硬化病变中血管细胞粘附分子-1(VCAM-1)的表达和阿托伐他汀的抗动脉粥样硬化作用。方法24只大耳白兔随机分为常规饲料组、胆固醇饲料组和阿托伐他汀组,饲养16周。实验前后检测(血清胆固醇TC)、甘油三酯(TG)、低密度脂蛋白(LDL),采用免疫组化和逆转录多聚酶链式反应(PCR)方法测定主动脉壁VCAM-1阳性表达百分比和mRNA的表达。结果阿托伐他汀显著降低TC和LDL水平(P<0.01)。胆固醇饲料组和阿托伐他汀组病变局部VCAM-1的阳性染色百分比和mRNA的表达量明显高于对照组(P<0.01),但阿托伐他汀组动脉粥样硬化病理改变较胆固醇饲料组明显减轻,VCAM-1阳性染色百分比及mRNA的表达量较胆固醇饲料组明显降低(P<0.01)。结论动脉粥样硬化的发生伴有血管细胞粘附分子的高度表达。阿托伐他汀不但有调脂作用,而且具有抑制粘附分子和抗动脉粥样硬化的作用。

Objective To investigate the expression of VCAM-1 in atherosclerotic rabbits and effects of atorvastatin in anti atherosclerosis. Methods Twenty four rabbits were randomly divided into control,cholesterol diet.and atorvastatin group,which were fed for 16 weeks. Serum TC ,TG,LDL were measured in 0 and 16 weeks in the three groups. After 16 weeks, the aortas were harvested for pathologic morphology observation,immunohistochemistry analysis of VCAM-1 positive percentage and the measurement of VCAM-1 mRNA using reverse transcription-polymerase chain reaction（RT-PCR） were performed. Results Atorvastatin greatly reduced TC and LDL （P〈0.01）. Atherosclerosis was formed in cholesterol diet and atorvastatin group ,the expressions of VCAM-1 and the measurement of VCAM-1 mRNA in cholesterol diet and atorvastatin group were higher than control （P〈 0.01 ）,but pathologic changes .intima thickness and intima/medium thickness ratio（I/M ） in atorvastatin group reduced than cholesterol diet group（P〈0. 01 ） ,the expressions of VCAM-1 and the measurement of VCAM-1 mRNA were lower than cholesterol diet group （P 〈 0. 01 ）. Conclusions VCAM-1 can be excessively expressed in atherosclerosis. Atorvastatin not only reduces cholesterole but also inhibits excessive expression of VCAM-1 and has the effect of anti-atherosclerosis.