甲基维生素B_(12)对拟血管性痴呆大鼠记忆功能及脑白质病理变化的影响

The effect of memory and cerebral white matter neuropathology changes of vascular dementia rats treated with methlcobalamine

目的观察甲基维生素B12对拟血管性痴呆大鼠记忆功能及脑白质病理变化的影响。方法反复前脑缺血制备大鼠拟血管性痴呆模型,造模后甲基维生素B12治疗1周、2周、4周时Morris水迷宫检测大鼠学习记忆功能、LFB染色观察白质髓鞘变化、电镜下观察少突胶质细胞、髓鞘超微结构,并与假手术组及对照组对比。结果治疗4周大鼠游迷宫时间明显短于对照组,造模2周、4周时LFB染色均可见白质髓鞘脱失,但治疗组明显较对照组轻。造模4周电镜下少突胶质细胞、髓鞘改变治疗组轻于对照组。结论甲基维生素B12能改善拟血管性痴呆大鼠记忆功能,并能减轻脑白质病理变化。

Objective To observe the effect of memory and cerebral white matter neuropathology changes of vascular dementia rats treated with methlcobalamine. Methods Vascular dementia rats models were made by clamping bilateral carotid arteries. Morris water maze detected learning and memory ability. LFB staining observed myelin changes in white matter ,electric microscope was used to observe the ultrastructure of internal capsule oligodendrocyte and myelin sheath before operation and at lw,2w,4w treated with methlcobalamine after operation. These results were all controlled with sham-operated groups and ischemia and operation groups. Results The average time for rats swimming at 4w in therapy group was obviously reduced compared with that of ischemia and operation groups, LFB staining of cerebral white matter at 2w,4w appeared demyelination in ischmia and operation groups,but the changes were lightened in therapy groups compared with that of ischemia and operation groups. The damages of oligodendrocyte and myelin sheath in internal capsule at 4w were lightened in therapy groups compared with that of isehemia and operation groups. Conclusion Methlcobalamine improves the learning and memory ability of vascular dementia rats and decreases white matter neurology damages.