RAPD图谱为依据的重要致病性暗色丝孢科真菌分子系谱学研究

Molecular phylogenetic studies on the medically important dematiaceous fungi based on randomly amplified polymorphic DNA finger prints

目的研究人类致病真菌裴氏着色霉、紧密着色霉、疣状瓶霉、皮炎瓶霉、卡氏枝孢霉、甄氏外瓶霉间的分子系谱格局。方法采用数值表相学方法,对上述6种30株重要致病性暗丝孢科真菌的随机扩增多态性DNA指纹图谱进行分析。结果得出其系谱格局为{P.verrucosa[(F.pedrosoi F.compacta)W.dermatitidis][E.jeanselmeiC.carrionii]}。W.dermatitidis种内高度同源性,E.jeanselmei种内高度异源性。结论显示F.pedrosoi和F.compacta、E.jeanselmei和C.carrionii,以及Fonsecaea和Wangiella之间有密切关系。关键词:暗色丝孢科;真菌;系谱学;

Randomly amplified polymorphic DNA （RAPD） finger prints in combination with numerical phonetic analysis was used to investigate the molecular phylogeny of 6 species of 30 strains of the medically important Dematiaceae, i.e. Fonsecaea pedrosoi, Fonsecaea compacta, Phialophora vrruucosa, Wangiella dermatitidis, Cladosporium carrionii and Exophiala jeanselmei. The cladistic pattern was described conveniently as that： t P, verrucosa [ （ F. pedrosoi F. compacta ） W, dermatitidis ] [ E, jeanselnei C. carrionnii ] } W. dermatitidis was monophyletic, while E. jeanselmei was polyphyletic. These results indicate that very close relationships exist between isolates of F. pedrosoi and F. compacta , E . jeanselmei and C. carrionii as well as Fonsecaea and Wangiella.