摘要
目的阐明多点接种真菌培养法在实验室诊断甲真菌病的作用和意义。方法采用多点接种真菌培养法和传统的点植培养法对两组甲真菌病患者的甲屑进行致病真菌的分离和鉴定,比较两种方法检出甲真菌病致病真菌的构成比例。结果试验组和对照组培养阳性率分别为为53.04%和37.50%(P=0.029)。致病菌分离鉴定结果:试验组皮肤癣菌49.21%,酵母菌36.51%,霉菌14.29%;对照组则分别为76.92%,20.51%和2.82%(P=0.001)。试验组共检出2例混合感染,对照组则无。结论多点接种法真菌培养可以提高对甲真菌病的病原菌的检出率,尤其是对非皮肤癣菌酵母菌和霉菌的检出率,且有助于发现甲真菌病的混合感染,但对非皮肤癣菌致病性的判定有待进一步研究。
Objective To study the role of multiple spots Inoculation of fungi in sabouraud agar for the diagnosis of onyehomycosis,especially evaluating pathogenic potential of non-dermatophytes and differentiating the mixed infection. Methods Pathogen of onyehomycosis was detected by multiple spots inoculation fungal culture and single spot inoculation, anti the isolated fungi resuits of the study group and the control group were compared. Results The positive rate was 53.04% in the study group and 37. 50% in the control group. ( P = 0. 029 ). The isolated fungi results of the study group were dermatophytes 49.21% , yeasts 36.51% and moulds l4.29% , and the control group were 76.92% ,20.51% and 2.82% ( P = 0. 001 ). Totally 2 mixed infection were identified from study group. Conclusion Multiple spots inoculation fungal culture could increase the fungal detection rate. and increase the non - dermatophyte and mould detection rate,and was helpful on detection and differentiation of the mixed infeetion of the onychomycosis,but more studies should be done on the criterion for the judgement of pathogenic potential of non-dermatophytes.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2005年第9期525-526,532,共3页
The Chinese Journal of Dermatovenereology