沙眼衣原体微量细胞培养和鉴定

Cell Microculture and Identification of Chlamydia Trachomatis

目的细胞培养沙眼衣原体,并应用四种方法对培养结果进行鉴定。方法将McCoy细胞在细胞培养板中培养至致密单层后,接种沙眼衣原体标准株和临床株,培养44～48h后,用卢戈氏碘液染色、Giemsa染色、单克隆荧光抗体和内源性质粒扩增四种方法鉴定培养结果。结果四种鉴定方法结果均呈阳性,证实沙眼衣原体培养成功。结论沙眼衣原体培养的成功将为该病原体的致病机理、免疫机制、药物敏感性检测和保护性疫苗等方面的研究奠定基础。

Objective To cuhure Chlamydia trachomatis（CT） in McCoy cell and to identify the pathogen by four methods. Methods After McCoy cell cuhured in cell cuhure cluster for 16 - 24 hours,the standard strain and wild strain of CT were inoculated and cultured for another 44 -48 hours. The existence of CT was identified by four methods. They were Logus iodine dying, Giemsa dying,mnnoclonal fluorescence antibody and plasmid PCR. Results Positive results were made by these four methods, which showed the success of CT culture. Conclusion Successful CT culture establishes good foundation for further research of CT including pathogenesis, immunologic mechanism, drug susceptibility test and protective vaccine.