前列腺素E_1在低钾右旋糖酐液供肺保存中的作用

Effect of Prostaglandin E_1 in Lung Preservation with LPD Solution

目的探讨前列腺素E1(PGE1)是否能改善低钾右旋糖酐液的肺保存效果。方法实验动物分为对照组(单纯肺动脉冲洗组)和实验组(肺动脉冲洗前经肺动脉注射PGE1组)。供肺经24 h保存后采用离体肺循环灌流模型再灌流60 min。灌流中观察肺动静脉血氧分压差(ΔPO2)、肺动静脉血二氧化碳分压差(ΔPCO2)、肺动脉压(PAP)、气道压(PAW)、静态肺顺应性(Cstat)的变化,并比较两组间肺重湿干比(W/D)。结果实验组肺动脉冲洗时间明显缩短,但冲洗末肺组织中仍残存血液成分。灌流中同对照组一样出现肺不张、肺水肿、肺出血,肺功能也未明确改善。结论前列腺素E1并不能改善低钾右旋糖酐液的供肺保存作用。建议在应用PGE1时为保证彻底冲洗,应加大冲洗液用量。

Objective To observe the effect of PGE1 in lung preservation with LPD solution. Methods 12 rabbits were evenly divided into control group and experimental group at random. Pulmonary artery was flushed with LPD solution during donor lung harvesting. PGE1 was administered through pulmonary artery prior to flushing in experimental group. All lungs were storaged at 4℃ for 24 hours, then reperfused for 60 minutes with an ex vivo isolated and circulated perfused lung model. Blood gas, PAP, PAW, Cstat, W/D were studies. Results There were no significant differences between the two groups in the changes of APO2, APCO2, PAW, Cstat, W/D. Shorter flush time was obtained in experimental group, but blood residues in some lungs were also noticed after flushing. Conclusion It does not clearly demonstrate that PGE1 can improve lung preservation with LPD solution in this study. We recommend much volume of flush solution be needed if PGE1 is administered.