石墨炉原子吸收法测定人红细胞膜结合硒

Determination of Binding Selenium in Human Red Erythrocyte Membrane by Graphite Furnace Atomic Absorption Spectrometry

本文采用镍－硝酸镁作基体改进剂，用石墨炉原子吸收法测定人红细胞膜结合硒，并对该方法进行了研究探索。在选定的实验条件下，该方法特征质量为１６．０ｐｇ／０．００４４Ａ．Ｓ，检出限为７．４ｐｇ。回收率为９２％－１０８％，相对标准偏差为８．９％，１０种共存元素含量在正常生理浓度范围内不干扰测定。经对样品测试分析，证明本方法准确、快速，满足分析要求。

A method for determination of binding selenium in human red erythrocyte membrane with Ni and Mg (NO_3)_2 as matrix modifier in graphite furnace AAS is described. Under the optimum condition,the characteristic mass m is 16. 0pg/0. 0044A. S,the detection limit is 7.4pg,the recovery is 92%-108 % ,the ralative standard deviation is less than 8. 9%.10 elements do not interfere with the determination of Se. The method had been used to analyse practical samples ,satisfactory results are obtained. It is simple and rapid.