糖化酵母在酒精工业中应用研究（Ⅰ）──去除INH1基因的酒精酵母的选育

THE STUDY AND APPLICATION OF SACCHAROMYCES DIASTATICUS(Ⅰ)──THE BREEDING OF DISTILLER'S YEAST WITH ELIMINATED INH1 GENE

二倍体耐高温酒精酵母经诱导生孢子，单孢子分离及营养缺陷型遗传标记的制作，获得了一株与亲本发酵性能相当且遗传性能稳定的单倍体菌株ＪＤ９－２２（α，ｓｔａ，ＩＮＨ１，ａｒｇ）。利用酵母的群体杂交方法，通过糖化酵母单倍体菌株２６０６７－４４（ａ，ＳＴＡ，ｉｎｈ，ａｄｅ）于ＪＤ９－２２杂交，选育去除了ＩＮＨ１基因的耐高温酒精酵母单倍体，解除了ＩＮＨ１基因对ＳＴＡ基因在酒精酵母细胞内表达的抑制。

Auxotrophic haploid JD9-22 strain of thermotolerent distiller's yeast was obtained by U. V. mutation. By hybridization JD9-22 (α,STA, inh, ade-) of Saccharomyces diastaticus, mass spore isolation, the segregation and recombinatin of STA gene, INH1 gene and the genetic markers, meiosis Z6-10(α,STA, inh, arg- ) strain with eliminated INH1 gene was selected.