IL-10、IL-18在重症急性胰腺炎肺损伤中的作用

Effect of IL-10 and IL-18 on Acute Lung Injury of Severe Acute Pancreatitis

目的探讨重症急性胰腺炎(SAP)中IL-10及IL-18的表达及其与SAP肺损伤的关系。方法将48只SD大鼠按随机数字表法均分为对照组和SAP组,动态定量测定肺湿重系数、腹水量、血清淀粉酶、血清IL-10及IL-18的变化和半定量RT-PCR检测肺组织中IL-10mRNA及IL-18mRNA的表达,并在光镜下观察胰腺及肺组织病理学改变。结果各时相SAP组肺湿重系数、腹水量、血清淀粉酶、血清IL-10及IL-18水平和肺组织中IL-10mRNA及IL-18mRNA的表达与对照组相比均明显增高(P<0.05,P<0.01)。SAP组大鼠血清中IL-18水平和肺组织IL-18mRNA表达水平均与肺湿重系数(r=0.68,P<0.01;r=0.72,P<0.01)及肺损伤病理评分(r=0.74,P<0.01;r=0.79,P<0.01)呈正相关,而血清中IL-10水平和肺组织IL-10mRNA表达水平均与肺湿重系数(r=-0.62,P<0.01;r=-0.69,P<0.01)及肺损伤病理评分(r=-0.66,P<0.01;r=-0.60,P<0.01)呈负相关。结论IL-18参与了SAP并发肺损伤的病理过程,而IL-10对肺损伤有保护作用。

Objective To investigate the role of interleukin-10（IL-10） and interleukin-18 （IL-18） in the pathogenesis of acute lung injury in experimental severe acute pancreatitis. Methods Forty-eight SD rats were divided into control group and SAP group by the random data table. The model of experimental severe acute pancreatitis was established by injection of 3.5 % sodium taurocholate into the bill-pancreatic duct. Lung wet weight index, ascities and level of serum amylase, IL-10 and IL-18 were quantitatively measured in different time. Intrapulmonary expressions of IL-10 mRNA and IL-18 mRNA were detected by semi-quantitative RT-PCR. The histopathology of pancreas and lung were observed under the light microscope. Results Lung wet weight index, ascities, level of serum amylase, IL-10 and IL-18, intrapulmonary expressions of IL-10 mRNA and IL-18 mRNA were significantly increased in SAP group （P〈0.01）. The level of serum IL-18 and intrapulmonary expression of IL-18mRNA are positively correlated with lung wet weight index （r=0.68,P〈0.01; r=0.72,P〈0.01） and lung injury score （r=0.74,P〈 0.01; r=0. 79, P〈0.01） respectively, whereas the level of serum IL-10 and intrapulmonary expression of IL-10 mRNA are negatively correlated with lung wet weight index（r=-0.62 ,P〈0.01; r= -0.69 ,P〈0.01） and lung injury score（r= -0.66,P〈0.01; r=-0.60,P〈0.01）. Conclusion IL-18 may play a key role in the pathogenesis of acute lung injury in experimental severe acute pancreatitis, and IL-10 exerts the protection role in this process.