酒精对体外肝细胞极性的影响

Effects of Ethanol on Polarization of Cultured Hepatocytes

目的观察酒精对培养肝细胞极性的影响。方法利用三明治构型培养成鼠肝细胞并观察酒精干预下其形态、膜区域蛋白特异性分布以及P450-b和白蛋白mRNA表达的变化,以确定酒精对肝细胞极性的影响。结果酒精干预后,肝细胞变扁平、多核、胞浆稀薄,肝板样结构消失;免疫组织化学染色证明肝细胞膜区域蛋白失去特异性分布;原位杂交显示加入酒精后只有较弱的P450-b和白蛋白mRNA阳性表达。结论酒精可导致肝细胞极性破坏,从而造成肝细胞损伤,本研究为在细胞水平研究酒精性肝病的发病机理提供了一个体外模型,具有较重要的临床意义。

Objective To investigate the effects of ethanol on polarization of cultured hepatocytes. Methods Sandwich hepatocytes were used to study the effects of ethanol on polarization of short-period cultured hepatocytes. Expression of albumin mRNA and P450-b mRNA,specific distribution of plasma membrance protein and the morphology were observed before and after ethanol was given. Results After treated ethanol, hepatocytes became flat and polynuclear, lts cellular plasma became watery and anatomical networks like board structure disappeared. Immunohistochemical method proved that the distribution of specific plasma membrane protein was damaged, and in situ hybridization studies demonstrated that the levels of albumin mRNA, P450-b mRNA expression decreased after ethanol was given. Conclusion Ethanol induces significant disturbance of cell polarization in cultured hepatocytes to result in injury. The current study is important for exploring the mechanism of alcohol liver disease from the cellular plane to provide a model.