脑膜炎球菌病（蒙古）

Establishment and Preliminary Application of a 16SrRNA Polymerase Chain Reaction Assay for Detection of Legionella Species

脑膜炎球菌病（蒙古）１９９４和１９９５年发生了流行性脑膜炎球菌病。在１９９４年１－４月期间总计报告疑似细菌性脑膜炎１７５４例，相应的总发病率为每１０万人口８０例，死亡１２２例［病死率（ＣＦＲ）为７％］。首都乌兰巴托市（人口为５９６０００）报告１０７０...

Abstract A 165rRNA polymerasse chain reaction CPCR) assay for the detection of Le- gionella Species was established. A 375 bp fragment of the 16SrRNA gene could be detected by amplifing genomic DNA of reference strains (Lp1-14, Lm, L1, Ld, Lb, Lg). The same result was also obtained by amplifing 5 identified strains isolated from China. The amplified products were verified by hybridization with 16SrRNA gene probes. However, 14 non-legionella strains could't produced a positive amplified fragment. Preparing bacterial genomic DNA by boiling method,the assay could detect 280cfu/ml water and 560 cfu/ml clinical bro