摘要
抗性遗传分析结果表明,转Cry1Ac基因棉选育抗性棉铃虫(Helicoverpa armigera(Hübner))对Bt毒素Cry1Ac的抗性遗传方式为不完全隐性,其回交后代(BC)用40μg.ml-1Cry1Ac的饲料饲喂5 d后,存活虫数∶死亡虫数的比例为1∶1(X2<X20.05);BtR与AFLP标记EaaMcta02连锁,位于第2条连锁群上EaaMcta02和EaaMcta03两个标记之间,遗传图距分别为10.40 cM和13.90 cM。本研究结果将为制定棉铃虫对转Bt基因棉的抗性监测及抗性治理方案提供一个依据。
The results of resistance genetical analysis showed that the CrylAc toxin resistance in resistant strain H. arrnigera selected by transgenic CrylAc cotton was incompletely recessive, and the backcross progeny segregated 1 ： 1 （X^2 〈 X0.05^2） for resistant vs. susceptible phenotypes, feeding diet with 40μg·ml^-1 Cry1Ac for 5 d. AFLP method was applied to map the resistant gene marker in H. armigera to transgenic CrylAc cotton. The results showed that 82 DNA markers were generated from 47 BC1 progenies by ten primer combinations, among which 12 markers were distributed into four linkage groups; BtR gene flanked with two AFLP marker, EaaMcta02 and EaaMcta03, on the 2nd linkage group in H. armigera, with 10. 40 cM and 13. 90 cM linkage distance; meanwhile, 68 markers were gained from 48 BC2 progenies with the same primer combinations, among which 29 markers clustered into nine linkage groups, and BtR gene couldn＇t be located on any group. The results also suggested that the number of amplified fragments depends on the primer combination and usually ranges in number from 50 to 100, and AFLP amplification product can be visualized by silver staining with sensitivity and resolution similar to these observed with radioactive labelling. The results provide a guidance in understanding, monitoring, and managing resistance to transgenic Bt cotton in H.armigera.
出处
《棉花学报》
CSCD
北大核心
2005年第5期269-274,共6页
Cotton Science
基金
国家自然科学基金资助项目(30270889)
