2,5-己二酮对大鼠神经组织神经丝含量的影响

Effect of 2,5-hexanedione on the content of neurofilament in rat nervous system

目的初步探讨正己烷中毒性周围神经病变中2,5己二酮(HD)对大鼠大脑、脊髓、坐骨神经中神经丝(NF)亚单位含量的影响。方法Wistar雄性大鼠分3组,2个染毒组,1个对照组,每组9只,经腹腔染毒HD,剂量分别为200和400mg/kg,连续8周(每周5d),分离大脑、脊髓、坐骨神经组织,匀浆,28000g、30min离心制备各组织上清和沉淀,采用十二烷基磺酸钠聚丙烯酰胺凝胶电泳(SDSPAGE)和WesternBlotting检测低分子量NF(NFL)、中分子量NF(NFM)、高分子量NF(NFH)的相对含量。结果大脑、坐骨神经上清中未检测到NFL、大脑上清中未能检测到NFM,其他NF亚单位的含量在沉淀和上清中均明显降低,下降为对照组的5%～77%,其中以NFM和坐骨神经组织中NF含量的降低更为明显。结论HD导致大鼠各神经组织中NF含量的降低,NF含量改变与正己烷中毒性周围神经病变的发生有关。

Objective To investigate the alterations of neurofilament （NF） in cerebrum, spinal cord and sciatic nerve of rats intoxicated with 2,5-hexanedione（HD） .Method HD was administered by intraperitoneal injection to Wistar rats with daily dosing of 200 and 400 mg/kg for continuous 8w （five times per week）. Cerebrum, spinal cord and sciatic nerve were excised and homogenized, then centrifuged at 28 000g for 30 min to prepare the supernatant and pellet sample. The relative levels of low molecular weight NF（NF- L）, medium molecular weight NF（NF-M）, and high molecular weight NF （NF-H） were determined by SDS-PAGE and Western Blotting. Results Except for supernatant NF-L in the cerebrum and sciatic nerve, and supernatant NF-M in the cerebrum were not detected, HD-intoxication was associated with dose-dependent reductions in NF subunit contents of the supernatant and pellet fractions, with the relative content 5% to 77% of the control proximately. Among the nervous tissues and three NF subunits, the decreases in the sciatic nerve and NF-M tended to he greater than the others. Concluslon HD-intoxication results in alterations in NF content, and the alterations are related to its peripheral neurotoxicity.