盐酸克伦特罗体外抑制大鼠胚胎肢芽细胞增殖和分化的研究

Study on inhibitory effect of clenbuterol on proliferation and differentiation of rat embryonic limb bud cells in vitro

目的探讨盐酸克伦特罗对大鼠胚胎肢芽细胞的影响。方法采用大鼠胚胎肢芽细胞培养方法,观察盐酸克伦特罗对大鼠胚胎肢芽细胞增殖和分化的影响。结果盐酸克伦特罗对大鼠胚胎肢芽细胞增殖、分化均有抑制作用;细胞增殖:y^=0.507-0.000 7x,r=-0.949(P<0.01);细胞分化:y^=87.63-0.286x,r=-0.945(P<0.01),存在明显的剂量—反应关系,并呈显著性负相关;胚胎肢芽细胞半数抑制细胞增殖浓度(IP50)为0.34μg/L,半数抑制分化浓度(ID50)为0.09μg/L,IP50/ID50=3.78。结论盐酸克伦特罗可抑制大鼠胚胎肢芽细胞增殖和分化作用。

Objective To study the effect of clenbuterol on limb bud cells of embryo of rats. Methods The micromass rat embryonic limb bud cell culture was used to determine the effects of elenbuterol on proliferation and differentiation of limb bud cells. Results Both the rate of proliferation and differentiation of rat embryonic limb bud ceils were decreased with a dose-effect relation. The concentration of cell survival or proliferation by 50% of the control （IP50） and the inhibitory concentration of the formation of differentiated foci by 50% of the control （ ID50 ） were shown to be 0.34 μg/L and 0.09 μg/L respeetively. The rate of IP50/ ID50 of clebuterol was 3.78. Conclusion Clenbuterol can inhibite the proliferation and differentiation of limb bud cells of rat embryo.