马铃薯卷叶病毒的RT-PCR快速检测

A rapid method for detecting potato leafroll virus by reverse transcription-polymerase chain reaction amplification

根据马铃薯卷叶病毒的外壳蛋白基因序列,设计合成了一对寡核苷酸引物。从感染马铃薯卷叶病毒(PLRV)的马铃薯叶片中提取出病毒RNA,进行cDNA合成并运用RT-PCR技术进行体外扩增,得到一条长度约627bp的特异PCR扩增产物,与理论设计的外壳蛋白基因大小一致,而对照未得到任何产物。从而建立了快速灵敏的PLRV检测方法,为PLRV的防治及检测提供了有效手段。

A pair of DNA primers were designed and synthesized based on the nucleotide sequence of the coat protein （CP） gene of potato leafroll virus （PLRV）. The PLRV RNA which was used for cDNA synthesis was directly extracted from virus-infected potato leaves. A specific PCR fragment about 627 bp was obtained by reverse transcription and polymerase chain reaction （RT-PCR） amplification, which had the expected length of PLRV CP gene, at the same time, no fragment was obtained from the control. Therefore, a rapid and sensitive RT-PCR detection system of PLRV was established, which provided an effective way to detect and control PLRV.