肾缺血再灌注后血清、脑脊液和脑组织中Ca^(2+)和超氧化物歧化酶活性变化的研究

Study on the change of calcium and superoxide dismutase in serum, cerebrospinal fluid and brain tissue after renal ischemia/referusion injury

目的通过测定肾缺血再灌注后血清、脑脊液和脑组织中Ca2+含量和超氧化物歧化酶(SOD)活性,探讨其在肾缺血再灌注损伤中的变化规律。方法20只健康新西兰兔随机分为对照组和肾缺血再灌注组(IR组),检测和比较缺血再灌注后24小时血清和脑脊液中尿素氮(BUN)含量,以及血清、脑脊液和脑组织中Ca2+含量和SOD活性。结果与对照组相比,在肾缺血再灌注后24小时IR组血清和脑脊液中的BUN显著升高,IR组脑脊液和脑组织中Ca2+含量显著升高而SOD活性均显著降低。结论肾缺血再灌注损伤后,脑脊液和脑组织中自由基生成增多,出现钙积聚,提示肾缺血再灌注损伤后神经系统功能的改变可能与其相关。

Objective：To explore the change mechanism of calcium and superoxide dismutase （SOD） in serum, cerebrospinal fluid and brain tissue after renal ischemia/ referusion injury. Methods ： Twenty healthy New Zealand rabbits were randomly divided into control group and renal ischemia/referusion injury group （IR group） .At the time point of referusion 24h after renal ischemia, the contents of BUN in serum and cerebrospinal fluid were determined. At the same time SOD activity and calcium content were evaluated respectively in serum, cerebrospinal fluid and brain tissue. Results： Compared with control group, the content of BUN was higher in serum and cerebrospinal fluid in IR group. In IR group the content of Ca^2＋ increased and SOD activity reduced in cerebrospinal fluid and brain tissue compared with control group. Conclusion： Renal ischemia/referusion injury can lead to free radical augment and calcium accumtdation in brain tissue and cerebrospinal fluid, which indicates that disftmction of nerve system may be related to calcium and free radical in cerebrospinal fluid and brain tissue.