大鼠外周血淋巴细胞和脾淋巴细胞HPRT基因突变的比较

Comparative Study on HPRT Gene Mutation in Rat Peripheral Blood Lymphocytes and Spleen Lymphocytes

目的克隆法研究放射性核素内照射诱发大鼠体细胞HPRT基因突变,比较不同体细胞HPRT基因突变频率。方法大鼠尾静脉注入晚期混合裂变产物,克隆法检测不同累积剂量的放射性核素内照射诱发的外周血淋巴细胞和脾淋巴细胞HPRT基因突变。结果外周血淋巴细胞和脾淋巴细胞的克隆效率均随内照射剂量的增加而降低,HPRT基因突变频率均随放射性核素内照射剂量的增加而增加。相同条件下,脾淋巴细胞HPRT基因突变比外周血淋巴细胞敏感。结论克隆法是一种敏感的检测辐射诱发HPRT基因突变的方法,脾淋巴细胞HPRT基因突变比外周血淋巴细胞敏感。

Objective To study and compare the HPRT gene mutation in rat soma cells induced by internal exposure to radionuclides using cloning method. Methods The radionuclides were injected into the rats via tail vein and the HPRT gene mutation were detected using cloning method. Results The clony efficiencies of rat peripheral blood lymphocytes and spleen lymphocytes both declined with the increase of accumulative dose, while the HPRT gene mutation frequency rose. Conclusion Cloning method is a relatively sensitive method to detect HPRT gene mutation induced by radiation, and the HPRT gene mutation of spleen lymphocytes is more sensitive than that in peripheral blood lymphocytes.