摘要
目的探讨褪黑素对缺血再灌注后大鼠肝脏NF-κB表达及iNOS的影响.方法采用大鼠部分肝缺血再灌注模型,将健康雄性SD大鼠78只随机分为假手术组(S)、缺血再灌注组(I/R)、褪黑素处理组(Mel)3组,分别检测标本中NF-κB表达和iNOS含量.结果S组无NF-κB阳性表达;I/R组和Mel组经历单纯的肝脏缺血后,NF-κB的表达与S组无差异(P＞0.05),而再灌注后两组均有不同程度的NF-κB表达增加,并均于6 h时点处达到各自峰值,Mel组于再灌注后各相应时点NF-κB的阳性表达率显著低于I/R组(P＜0.01).再灌注后0～1 h,3组iNOS活力无差异(P＞0.05),此后,I/R组iNOS活力随再灌注时间的延长而较S组显著上升(P＜0.01),其高峰亦在9 h时点处出现,Mel组再灌注1 h后各时点iNOS活力均较I/R组显著下降(P＜0.01).结论褪黑素可以有效下调肝脏I/R过程中NF-κB的表达,抑制iNOS的活力,发挥其对肝脏缺血再灌注损伤的保护作用.
Objective To explore the influence of in rat liver after ischemia and reperfusion. Methods melatonin on the expression of NF-κB and iNOS With a rat model of partial hepatic ischemia and reperfusion, 78 healthy male SD rats were randomized into 3 groups： sham-operation group（s）, ischemia/reperfusion group（I/R）, melatonin group（Mel）. The expression of NF-κB was evaluated and the measurement of iNOS was carried out. Results There was no expression of NF-κB in S group; After ischemia without reperfusion, the expression of NF-κB in both I/R group and Mel group was no statistic significance compared with S group（P 〉0.05）, however, the expression of NF-κB in both I/ R group and Mel group increased and reached the top at 6 h time point during the reperfusion period. The expression of NF-κB in Mel group were significantly lower than that in the I/R group at each corresponding time point during reperfusion （P 〈 0.01）. 0-1 h after reperfusion, there was no difference among three groups （P〉0.05）. At each following time point, the activity of iNOS in I/R group was much higher than that in the S group（P〈0.01）and reached the top at 9 h time point. The activity of iNOS in Mel group was significantly lower than that in the I/R group at each corresponding time point after 1 h（P 〈 0.01）. Conclusion Melatonin can down-regulate the expression of NF-κB and depress the activity of iNOS during the ischemia and reperfusion period and protect the liver from ischemia and reperfusion injury.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第4期563-566,共4页
Suzhou University Journal of Medical Science
