摘要
目的观察血管生成抑制剂联合放射治疗对大肠癌肝转移模型的治疗作用。方法采用BALB/cAnude裸小鼠,LS174T人大肠癌细胞株,脾脏切除脾脏种植法建立裸鼠大肠癌肝转移模型。并给予血管生成抑制剂2-ME及放射治疗,观察肝转移生长抑制及肿瘤组织微血管生成的抑制作用。结果脾切除脾脏种植法建立大肠癌肝转移模型的成功率达100%;种植后4周2-ME联合放射治疗组肝转移灶平均重量53.6±4.7 mg,而对照组为173.9±11.6 mg;微血管密度分别为8.4±1.7和41.2±6.3。结论2-ME联合放射治疗对肝转移灶的生长产生明显的抑制作用,抑制肿瘤微血管的形成是其有效性的机制之一。
Objective To observe the therapeutic effect of angiogensis inhibitor combined with radiotherapy on liver metastasis model of colon cancer. Methods Nude mice liver metastasis model of colon cancer was established with human colon cancer cells line （LS174T） inoculated into mice＇ spleen and followed by splenectomy. Angiogenesis inhibitor 2-ME and radiotherapy were administered afterwads. The growth inhibition effect on metastases and neovessel was examined. Results The incidences of liver metastasis was 100% in this intrasplenic injection model. The mean weight and mi- crovessel density 4 weeks after inoculation were 53.6 ± 4.7 rag, 8.4±1.7 in treatment group as compared to 173.9 ± 11.6 rag, 41.2 ± 6.3 in control group respectively. Conclusion 2-ME combined with radiotherapy has significant inhibition on the growth of liver metastases. Angiogensis inhibition is one of the mechanism of its efficiency.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第4期574-576,共3页
Suzhou University Journal of Medical Science
