精浆果糖定量检测试剂盒性能评价

A performance evaluation of quantitative assay kits of seminal plasma fructose

目的将两种国产同类试剂与WHO推荐吲哚法试剂性能进行对比分析.方法通过干扰试验、回收试验、重复性实验、对比试验等对3种试剂方法学进行评价.结果吲哚法、改良吲哚法、酶法干扰率依次为-7.6%～5.8%、-5.2%～4.9%、-2.5%～0.9%,平均回收率依次为76.2%～80.5%、77.5%～82.3%、95.5%～97.5%,重复性实验CV%依次为5.3%～7.8%、3.9%～5.3%、2.0%～3.4%,对比试验3种方法任一两两相比均有显著性差异.结论酶法是理想的方法学,而改良吲哚法优于吲哚法.

Objective To compare the performance of quantitative assay kits of seminal plasma fructose between two kinds of domestic reagents with enzyme method and modified indole method respectively and the reagent with indole method recommended by WHO. Methods The type of fructose kits with different methods were assessed by interfering experiment, recovery experiment, repeating experiment and contrasting experiment. Results For Indole method, modified indole method and enzyme method, the interfering rate is -7.6%-5.8%, -5.2%,-4.9% and -2.5%,4）.9% respectively, the average recovery rate is 76.2%-80.5%,77.5%-82.3% and 95.5%-97.5% respectively, CV% of repeating experiment is 5,3%-7.8%,3.9%-5.3% and 2.0%-3.4% respectively. There is obvious difference between each two. Conclusion The kit with enzyme method is an ideal, and the kit with modified indole method is better than one the idole method.