鸡大肠杆菌荚膜多糖──蛋白质抗原免疫原性的研究

STUDIES ON THE IMMUNOGENICTTY OF ANTIGENS OF CHICKEN PATHOGENIC Escherichia coli CAPSULAR POLYSACCHARIDES-PROTEIN CONJUGATES

用碳化二亚胺作为交联剂将荚膜多糖与载体蛋白质交联后加入蜂胶佐剂制成疫苗免疫鸡只。７周龄同源菌株攻毒结果表明，荚膜多糖－破伤风类毒素苗组鸡有很好的保护效果且试验重复性好；在免疫后３个月的同源攻毒保护指数也达１３／２０以上。但对异源菌株的攻毒没有明显保护效果。该研究采用微量法间接血凝试验检测了免疫鸡抗体水平。

Conjugation of capsular polysaccharides with carrier protein was carried out with N’-ethyl-N'-(3-dimethylaminopropyl)carbodiimde(EDC)as the coupling agent. The mean lesion score values of capsular polysaccharides(CPS)-tetanus toxoid OT)(CPS-TT)groups showed that they provided significant protection against chal-lenge with the same serotype strain in 7-week-old broilers.Moreover,the CPS-TT groups still exhibited more than 13/20 protection indiees three months post- vaccination. All broilers challenged with different serotype strains could not be pro- tected.The antibody levels of chickens indueed by vaccines were measured with the microplate indirect hemagglutination test.